By Lars Bjorndahl, David Mortimer, Christopher L. R. Barratt, Jose Antonio Castilla, Roelof Menkveld, Ulrik Kvist, Juan G. Alvarez, Trine B. Haugen
This useful, largely illustrated guide covers the techniques which are undertaken in andrology and paintings laboratories to examine and examine male-factor infertility, and to organize spermatozoa to be used in assisted belief remedy. The content material is gifted as short, authoritative overviews of the correct organic heritage for every quarter, plus special, step by step descriptions of the suitable analytical strategies. each one technical part contains pertinent quality controls issues, in addition to the optimal presentation of effects. as well as the great 'basic' semen research, incorporating cautious research of sperm morphology, the guide presents proven strategies for using computer-aided sperm research and sperm practical evaluate. through the instruction manual the translation of laboratory ends up in the scientific context is highlighted, and secure laboratory perform is emphasised. it really is a useful source to all scientists and technicians who practice diagnostic checking out for male-factor infertility.
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Additional resources for A Practical Guide to Basic Laboratory Andrology
Ca2+]i signalling in sperm – making the most of what you’ve got. Nat Cell Biol 2007; 9: 235–42. 36. Florman HM, Jungnickel MK, Sutton KA. Regulating the acrosome reaction. Int J Dev Biol 2008; 52: 503–10. 37. Raterman D, Springer MS. The molecular evolution of acrosin in placental mammals. Mol Reprod Dev 2008; 75: 1196–207. 38. Mortimer ST. A critical review of the physiological importance and analysis of sperm movement in mammals. Hum Reprod Update 1997; 3: 403–39. 39. Schatten G. The centrosome and its mode of inheritance: the reduction of the centrosome during gametogenesis and its restoration during fertilization.
Assessments that follow measurement of the ejaculate volume are best done after full liquefaction of the sample, followed by the microscopic investigations. 1 (above). Principle Even before the coagulated sample has liqueﬁed, the volume can be determined by weighing, provided that the sample collection container has been weighed before the sample was collected. 1 g, then all containers can be assumed to have the same weight and then it is not necessary to weigh each one before use. For every new batch of containers, a set of 30 should be tested to establish the consistency of their weight for that speciﬁc batch.
This situation calls for proper design and equipping of the semen collection room, and for situations that might cause stress for the men, such as long waiting times, embarrassing encounters, and disturbing noise from outside the room, to be avoided. Duration of stimulation can be important for the outcome, and men should be allowed ample time so as not to feel rushed to produce their samples . In general, patients are expected to collect a semen sample for investigation after between 2 and 7 days of ejaculatory abstinence.
A Practical Guide to Basic Laboratory Andrology by Lars Bjorndahl, David Mortimer, Christopher L. R. Barratt, Jose Antonio Castilla, Roelof Menkveld, Ulrik Kvist, Juan G. Alvarez, Trine B. Haugen