Read e-book online Advances in Stem Cell Therapy: Bench to Bedside PDF

By Nagwa El-Badri

ISBN-10: 3319291475

ISBN-13: 9783319291475

ISBN-10: 3319291491

ISBN-13: 9783319291499

The booklet stories the most methods for iteration of differentiated cells from quite a few varieties of stem cells, together with embryonic, placental and twine blood stem cells via marrow, adipose tissue and dental pulp. The publication starts off with an summary of experimental protocols utilized to generate insulin secreting cells, neural cells, center cells, and different tissue particular cells ex vivo and in experimental animals. this can be by means of exhaustive evaluation of scientific trials in those pathologies. It keeps with a comparability of the advantages of profitable transplantation in people as opposed to animal experimentation, and highlights the main promising medical functions within the box. distinctive chapters are dedicated to the subject of tissue engineering and glossy artificial and organic scaffolds. it truly is crucial studying for scientists and researchers in tissue engineering and stem cellphone learn in addition to clinicians who're eager about constructing or checking out stem mobilephone therapies.

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Diabetes. 2013;62(7):2588–94. 66. Wang S, Li Y, Zhao J, Zhang J, Huang Y. Mesenchymal stem cells ameliorate podocyte injury and proteinuria in a type 1 diabetic nephropathy rat model. Biol Blood Marrow Transplant. 2013;19(4):538–46. 67. Huang YC, Leung VY, Lu WW, Luk KD. The effects of microenvironment in mesenchymal stem cell-based regeneration of intervertebral disc. Spine J. 2013;13(3):352–62. 68. Venkatesan V, Madhira SL. Promise(s) of using mesenchymal stem cells in reproductive disorders.

For directed differentiation, many protocols were evaluated using culture media rich in glucose [41, 42]. Initial experiments used cells of murine origin 2 Stem Cell Therapy in Diabetes Mellitus 23 Fig. 1 Immunofluorescence staining of differentiated HBM-MSCs (a selected field). (a) Positive staining for intracytoplasmic insulin granules (green) with counterstaining for DAPI (blue). Positive staining for c-peptide (red) with counterstaining for DAPI (blue). Electronic merge of insulin and c-peptide staining.

5. Suciu S, Mandelli F, de Witte T, Zittoun R, Gallo E, Labar B, et al. Allogeneic compared with autologous stem cell transplantation in the treatment of patients younger than 46 years with acute myeloid leukemia (AML) in first complete remission (CR1): an intention-to-treat analysis of the EORTC/GIMEMAAML-10 trial. Blood. 2003;102:1232–40. 6. Alyea EP, Kim HT, Ho V, Cutler C, DeAngelo DJ, Stone R, et al. Impact of conditioning regimen intensity on outcome of allogeneic hematopoietic cell transplantation for advanced acute myelogenous leukemia and myelodysplastic syndrome.

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Advances in Stem Cell Therapy: Bench to Bedside by Nagwa El-Badri


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