Download e-book for kindle: Applications of Flow Cytometry in Stem Cell Research and by Awtar Krishan

By Awtar Krishan

ISBN-10: 0470543981

ISBN-13: 9780470543986

ISBN-10: 0470631112

ISBN-13: 9780470631119

A much-needed primer at the use of laser stream cytometry for stem telephone analysis

Laser stream cytometry is a strong instrument for swift research of cells for marker expression, cellphone cycle place, proliferation, and apoptosis. although, no assets in particular handle using this technique for the research of stem cells; this is often specially vital as stem cellphone research includes really expert equipment and marking approaches in line with particular features similar to marker expression, telephone measurement, drug shipping, and efflux of the stem cells.

Now, this ebook reports those systems, discusses the technology at the back of them, and gives real-world examples to demonstrate the usefulness of the equipment. It brings jointly world-class specialists in pathology, biophysics, immunology, and stem cellphone examine, who draw upon their huge event with the tools and express examples of excellent facts to aid advisor researchers within the correct path.

bankruptcy insurance comprises:

  • Stem mobile research and sorting utilizing facet inhabitants
  • Flow cytometry within the research of proliferation and apoptosis
  • Stem cellphone biology and alertness
  • Identification and isolation of very small embryonic-like stem cells from murine and human specimens
  • Hematopoietic stem cells—issues in enumeration
  • Human embryonic stem cells: long term tradition and cardiovascular differentiation
  • Limbal stem cells and corneal regeneration
  • Flow cytometric sorting of spermatogonial stem cells
  • Breast melanoma stem cells
  • Stem mobile marker expression in cells from physique hollow space fluids

This e-book is a vital source for all graduate scholars, practitioners in constructing nations, libraries and publication repositories of universities and learn associations, and person researchers. it's also of curiosity to laboratories engaged in stem phone learn and use of stem cells for tissue regeneration, and to any association dealing in stem mobilephone and tissue regeneration research.Content:
Chapter 1 fundamentals of circulate Cytometry (pages 1–12): H. Krishnamurthy and L. Scott Cram
Chapter 2 sensible concerns for stream Cytometric Sorting of Stem Cells (pages 13–23): Geoffrey W. Osborne
Chapter three Stem telephone research and Sorting utilizing aspect inhabitants research (pages 25–43): William Telford
Chapter four circulation Cytometry within the learn of Proliferation and Apoptosis (pages 45–60): Michael G. Ormerod and Ronald M. Hamelik
Chapter five stream Cytometric research of Drug shipping and Efflux in Stem Cells (pages 61–74): Awtar Krishan and Ronald M. Hamelik
Chapter 6 Stem mobilephone Biology and alertness (pages 75–89): Swapnil Totey, Rajarshi buddy, Murali Krishna Mamidi, Vijayendran Govindasamy and Satish Totey
Chapter 7 id and Isolation of Very SmaU Embryonic?like Stem Cells from Murine and Human Specimens (pages 91–101): Ewa ok. Zuba?Surma, Dong?Myung Shin, Habella Klich, Janina Ratajczak, Magda Kucia and Mariusz Z. Ratajczak
Chapter eight digital quantity of Hematopoietic Stem Cells (pages 103–114): Siddharth Sharma and Awtar Krishan
Chapter nine Hematopoietic Stem Cells: matters in Enumeration (pages 115–134): Michael Keeney and D. Robert Sutherland
Chapter 10 Embryonic Stem Cells: improvement and Characterization (pages 135–159): Vijay Bhaskar R. Konala, Villoo Morawala?Patell and Aparna Khanna
Chapter eleven Human Embryonic Stem Cells: Long?Term tradition and Cardiovascular Differentiation (pages 161–173): Maneesha Inamdar
Chapter 12 Mesenchymal Stromal Cells and Their scientific purposes (pages 175–188): Jyoti Kode and Vivek Tanavde
Chapter thirteen Circulating grownup Stem Cells of Hematopoietic beginning for Vascular and Neural Regeneration (pages 189–209): Lissy ok. Krishnan
Chapter 14 move Cytometric Characterization of Neural Progenitors Derived from Human Pluripotent Stem Cells (pages 211–222): Raj R. Rao, Sujoy okay. Dhara, Shilpa Iyer and David W. Machacek
Chapter 15 Limbal Stem Cells and Corneal Regeneration (pages 223–240): Geeta ok. Vemuganti, Murali Mohan Sagar Baila and Shubha Tiwari
Chapter sixteen stream Cytometric Sorting of Spermatogonial Stem Cells (pages 241–250): B. S. Srinag, J. M. Kalappurakkal, G.H. Mohan and H. Krishnamurthy
Chapter 17 Breast melanoma Stem Cells (pages 251–270): Devaveena Dey and Annapoorni Rangarajan
Chapter 18 Tumor Stem cellphone Marker Expression in Cells from physique hollow space Fluids (pages 271–277): Awtar Krishan, Deepti Sharma and Ronald M. Hamelik

Show description

Read or Download Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration PDF

Best cell biology books

New PDF release: Neurogenesis in the Adult Brain I: Neurobiology

The invention of grownup neurogenesis brought on a paradigm shift within the neurosciences. For greater than a hundred years, it used to be believed that grownup neurons don't regenerate. Joseph Altman and Fernando Nottebohm discovered evidence on the contrary and altered the process heritage. Their learn, integrated right here, presents the rules of the sphere.

New PDF release: Plant Tissue Culture, Third Edition: Techniques and

Plant Tissue tradition, 3rd variation builds at the school room established, viewers confirmed handbook that has guided clients via winning plant culturing A. tumefaciens mediated transformation, infusion expertise, the most recent info on media parts and guidance, and regeneration and morphogenesis besides new workouts and diagrams offer present info and examples.

Download e-book for iPad: Video Microscopy by Greenfield Sluder; D. E. Wolf

Video microscopy is used largely in lots of lifestyles and biomedical technology disciplines at the present time, and is an invaluable device for either phone biologists and scholars. This booklet provides tips on how to song the dynamic adjustments that ensue within the constitution of dwelling cells and in reconstituted arrangements utilizing video and electronic imaging microscopy.

New PDF release: Cancer Stem Cells. Targeting the Roots of Cancer, Seeds of

Melanoma Stem Cells: focusing on the Roots of melanoma, Seeds of Metastasis, and resources of treatment Resistance introduces the elemental options and complex figuring out of melanoma stem cells, protecting basic overviews, organ-specific identifications, and their attribute mechanisms. The ebook additionally explores leading edge healing concepts in preclinical and medical trials to focus on melanoma stem cells, get rid of the roots of melanoma, dispose of the seeds of metastasis, triumph over the resistance of treatments, and give a contribution to the eradication of melanoma.

Additional resources for Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration

Sample text

Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells. J Cell Sei 118:1715-1724. Burkert J, Otto WR, Wright NA. 2008. Side populations of gastrointestinal cancers are not enriched in stem cells. J Pathol 214:564-573. Cabana R, Frolova EG, Thomas RA, Krishan A, Telford WG. 2006. The minimal instrumentation requirements for Hoechst side population analysis: stem cell analysis on low-cost flow cytometry platforms. Stem Cells 24:2573-2581.

Although this is also true for stem cells, there are subtle differences in the approach that need to be taken to fully evaluate the effect of sorting on cells. For example, sorting stem cells from identical preparations at two different pressures can lead to the number of cells being recovered from each sample being identical and yet the long-term capacity of the sorted cells being quite different. Methods of evaluating the capacity of cells following sorting relate to their proliferative ability and for those cells that grow as colonies in media containing growth factors, the ability of the cells to form normal colonies or groups of cells.

Ocular surface epithelia contain ABCG2-dependent side population cells exhibiting features associated with stem cells. J Cell Sei 118:1715-1724. Burkert J, Otto WR, Wright NA. 2008. Side populations of gastrointestinal cancers are not enriched in stem cells. J Pathol 214:564-573. Cabana R, Frolova EG, Thomas RA, Krishan A, Telford WG. 2006. The minimal instrumentation requirements for Hoechst side population analysis: stem cell analysis on low-cost flow cytometry platforms. Stem Cells 24:2573-2581.

Download PDF sample

Applications of Flow Cytometry in Stem Cell Research and Tissue Regeneration by Awtar Krishan


by Ronald
4.4

Rated 4.88 of 5 – based on 27 votes